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eCAM Advance Access published online on December 5, 2006
eCAM, doi:10.1093/ecam/nel097
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© 2006 The Author(s).
Received February 22, 2006
Accepted October 30, 2006

Original Article

Vascular Pharmacology of Mokuboito (Mu-Fang-Yi-Tang) and Its Constituents on the Smooth Muscle and the Endothelium in Rat Aorta

Seiichiro Nishida 1 and Hiroyasu Satoh 1 *

1 Department of Pharmacology, Division of Crude and Herbal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan

* To whom correspondence should be addressed.
Hiroyasu Satoh, E-mail: hysat{at}naramed-u.ac.jp


  Abstract

Pharmacological actions of Mokuboito and its constituents (Sinomenium acutum and sinomenine) on rat aorta were examined. Mokuboito and S. acutum at lower concentrations (0.03-1 mg ml-1) contracted the non-loaded aorta, but at higher concentrations (1-3 mg ml-1), reversed to dilate it. The vasoconstriction was blocked by phentolamine (10 µM). Sinomenine failed to exhibit the vasoconstriction. On the other hand, Mokuboito and S. acutum dilated the NE (5 µM)-induced vasoconstriction: at 3 mg ml-1, by 98.9 ± 2.5% (n = 6, P < 0.01) and 97.0 ± 4.8% (n = 6, P < 0.01). Vasorelaxation induced by Mokuboito and S. acutum was attenuated by indomethacin, L-NMMA and nicardipine. Propranolol decreased the vasorelaxation induced by Mokuboito, but not by S. acutum. Sinomenine also relaxed the constriction and at 100 µM, by 68.8 ± 5.1% (n = 7, P < 0.01). This vasorelaxation was attenuated by indomethacin, L-NMMA and nicardipine, and also by propranolol. Therefore, these results indicate that Mokuboito and its constituents exert both vasodilating actions mediated by endothelium-dependent mechanisms (PGI2 and NO from endothelium) and by endothelium-independent mechanisms (Ca2+ influx control on smooth muscle cells). Simultaneously, Mokuboito and S. acutum cause the vasoconstrictions mediated through {alpha}-adrenoceptor stimulation, but not sinomenine. Also, Mokuboito and sinomenine possess {beta}-adrenoreceptor stimulating action, but not S. acutum.

Keywords: {alpha}- and {beta}-adrenoceptors; Ca2+ channel; EDRF; endothelium; Mokuboito; PGI2; rat aorta; sinomenine; Sinomenium acutum; vasodilation.
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