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eCAM Advance Access published online on December 1, 2006
eCAM, doi:10.1093/ecam/nel093
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© 2006 The Author(s).
Received June 18, 2006
Accepted October 13, 2006

Original Article

Anti-Inflammatory Activity of the Methanol Extract of Moutan Cortex in LPS-Activated Raw264.7 Cells

Seung-Chul Chun 1, Seon Young Jee 1, Sang Gon Lee 1, Sook Jahr Park 1, Jong Rok Lee 1, and Sang Chan Kim 1 *

1 College of Oriental Medicine and Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea

* To whom correspondence should be addressed.
Sang Chan Kim, E-mail: sckim{at}dhu.ac.kr


  Abstract

Moutan Cortex (MCE) has been used in traditional medicine to remove heat from the blood, promote blood circulation and alleviate blood stasis. This study was conducted to evaluate the effects of MCE on regulatory mechanisms of cytokines and nitric oxide (NO) involved in immunological activity of Raw264.7 cells. Cells were pretreated with methanolic extracts of MCE, and further cultured for an appropriate time after lipopolyssacharide (LPS) addition. During the entire experimental period, 0.1 and 0.3 mg ml-1 of MCE had no cytotoxicity. In these concentrations, MCE inhibited the production of NO and prostaglandin E2 (PGE2), the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor of {kappa}B{alpha} (p-I{kappa}B{alpha}), and the activation of nuclear factor {kappa}B (NF-{kappa}B). MCE also reduced the concentration of tumor necrosis factor-{alpha} (TNF-{alpha}), interleukin-1{beta} (IL-1{beta}) and interleukin-6 (IL-6) in the Raw264.7 cells that were activated by LPS. These results demonstrate that MCE has anti-inflammatory effects through the inhibition of iNOS and COX-2 expression by suppressing the phosphorylation of I-{kappa}B{alpha} and the activation of NF-{kappa}B.

Keywords: LPS; Moutan Cortex; NF-{kappa}B; nitric oxide.
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