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eCAM Advance Access published online on September 11, 2006
eCAM, doi:10.1093/ecam/nel059
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© 2006 The Author(s).
Received March 12, 2006
Accepted August 10, 2006

Original Article

Delayed Cell Cycle Progression and Apoptosis Induced by Hemicellulase-Treated Agaricus blazei

Masaki Kawamura 1 * and Hirotake Kasai 2

1 Department of Alternative Medicine and Bioregulation, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan
2 Department of Microbiology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

* To whom correspondence should be addressed.
Masaki Kawamura, E-mail: masakika{at}yamanashi.ac.jp


  Abstract

We examined the effects of hemicellulase-treated Agaricus blazei (AB fraction H, ABH) on growth of several tumor cell lines. ABH inhibited the proliferation of some cell lines without cytotoxic effects. It markedly prolonged the S phase of the cell cycle. ABH also induced mitochondria-mediated apoptosis in different cell lines. However, it had no impact on the growth of other cell lines. ABH induced strong activation of p38 mitogen-activated protein kinase (MAPK) in the cells in which it evoked apoptosis. On the other hand, ABH showed only a weak p38 activation effect in those cell lines in which it delayed cell cycle progression with little induction of apoptosis. However, p38 MAPK-specific inhibitor inhibited both ABH-induced effects, and ABH also caused apoptosis in the latter cells under conditions of high p38 MAPK activity induced by combined treatment with TNF-{alpha}. These results indicate that the responsiveness of p38 MAPK to ABH, which differs between cell lines, determines subsequent cellular responses on cell growth.

Keywords: antiproliferation; cancer; cell lines; fungus; p38 MAPK.
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