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eCAM Advance Access first published online on August 30, 2006
This version published online on September 14, 2006
eCAM, doi:10.1093/ecam/nel057
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© 2006 The Author(s).
Received December 16, 2005
Accepted July 25, 2006

Original Article

Use of Magnetic Nanoparticles to Visualize Threadlike Structures Inside Lymphatic Vessels of Rats

Hyeon-Min Johng 1, Jung Sun Yoo 1, Tae-Jong Yoon 2, Hak-Soo Shin 1, Byung-Cheon Lee 3, Changhoon Lee 1, Jin-Kyu Lee 2, and Kwang-Sup Soh 1 *

1 Biomedical Physics Laboratory, FPRD, School of Physics and Astronomy, Seoul National University, Seoul, Korea
2 Materials Chemistry Laboratory, School of Chemistry, Seoul National University, Seoul, Korea
3 Biomedical Physics Laboratory, FPRD, School of Physics and Astronomy, Seoul National University, Seoul, Korea; Research Institute of Basic Sciences, Seoul National University, Seoul, Korea

* To whom correspondence should be addressed.
Kwang-Sup Soh, E-mail: kssoh{at}phya.snu.ac.kr


  Abstract

A novel application of fluorescent magnetic nanoparticles was made to visualize a new tissue which had not been detectable by using simple stereomicroscopes. This unfamiliar threadlike structure inside the lymphatic vessels of rats was demonstrated in vivo by injecting nanoparticles into lymph nodes and applying magnetic fields on the collecting lymph vessels so that the nanoparticles were taken up by the threadlike structures. Confocal laser scanning microscope images of cryosectioned specimens exhibited that the nanoparticles were absorbed more strongly by the threadlike structure than by the lymphatic vessels. Further examination using a transmission electron microscope revealed that the nanoparticles had been captured between the reticular fibers in the extracellular matrix of the threadlike structures. The emerging technology of nanoparticles not only allows the extremely elusive threadlike structures to be visualized but also is expected to provide a magnetically controllable means to investigate their physiological functions.

Keywords: acupuncture meridian; Bonghan duct; lymph; nanoparticle; transmission electron microscope.
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