eCAM Advance Access published online on June 21, 2006
eCAM, doi:10.1093/ecam/nel037
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1 Division of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brigadier SK Mazumdar Marg, Delhi 110054, India
* To whom correspondence should be addressed. We have evaluated the effect of variation in aryl-tetralin lignans on the radioprotective properties of Podophyllum hexandrum. Two fractionated fractions of P. hexandrum [methanolic (S1) and chloroform fractions (S2)], with varying aryl-tetralin lignan content were utilized for the present study. The peroxyl ion scavenging potentials of S1 and S2 were found to be comparable [i.e. 45.88% (S1) and 41% (S2)] after a 48 h interval in a time-dependent study, whereas in a 2 h study, S2 exhibited significant (P < 0.05) antioxidant activity in different metal ion + flux states. In the aqueous phase, S2 exhibited non-site-specific reactive oxygen species scavenging activity, i.e. 73.12% inhibition at 500 µg ml-1. S1 exhibited 58.40 ± 0.8% inhibition (at 0.025 µg ml-1) of the formation of reactive nitrite radicals, comparable to S2 (52.45 ± 0.825%), and also showed 45.01% site-specific activity (1000 µg ml-1), along with significant (P < 0.05) electron donation potential (50-2000 µg ml-1) compared to S2. Such activities of S1 could be attributed to the significantly (P < 0.05) higher levels of podophyllotoxin
Received February 5, 2006
Accepted May 19, 2006
Original Article
Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
Raman Chawla 1,
Rajesh Arora 1,
Shikha Singh 1,
R. K. Sagar 1,
Rakesh Kumar Sharma 2 *,
R. Kumar 1,
A. Sharma 1,
R. P. Tripathi 1,
S. C. Puri 3,
H. A. Khan 4,
A. S. Shawl 5,
P. Sultan 5,
Tej Krishan 5,
and
G. N. Qazi 3
2 Division of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brigadier SK Mazumdar Marg, Delhi 110054, India; Defence Research Laboratory (DRDO), Tejpur, Assam, India
3 Natural Products Chemistry Division, Regional Research Laboratory (CSIR), Jammu Tawi 180001, India
4 Department of Medical Elementology and Toxicology, Jamia Hamdard, Hamdard Nagar, Delhi 110062, India
5 Regional Research Laboratory (CSIR), Field Station, Bonera, Srinagar, India
Rakesh Kumar Sharma, E-mail: rksharmadrl{at}yahoo.com
Abstract 
-D-glucopyranoside (16.5 times) and demethyl podophyllotoxin glucoside (2.9 times) compared with S2. Together, these findings clearly prove that aryl-tetralin lignan content influences the radiation protective potential of the Podophyllum fractions to a great extent.
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