eCAM Advance Access published online on June 8, 2006
eCAM, doi:10.1093/ecam/nel032
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1 Philadelphia Biomedical Research Institute, King of Prussia, PA 19406
* To whom correspondence should be addressed. We investigated whether ‘Ki-energy’ (life-energy) has beneficial effects on mitochondria. The paradigm we developed was to keep isolated rat liver mitochondria in conditions in which they undergo heat deterioration (39°C for 10 min). After the heat treatment, the respiration of the mitochondria was measured using a Clarke-type oxygen electrode. Then, the respiratory control ratio (RC ratio; the ratio between State-3 and State-4 respiration, which is known to represent the integrity and intactness of isolated mitochondria) was calculated. Without the heat treatment, the RC ratio was >5 for NADH-linked respiration (with glutamate plus malate as substrates). The RC ratio decreased to 1.86-4.36 by the incubation at 39°C for 10 min. However, when Ki-energy was applied by a Japanese Ki-expert during the heat treatment, the ratio was improved to 2.24-5.23. We used five preparations from five different rats, and the significance of the differences of each experiment was either P < 0.05 or P < 0.01 (n = 3-5). We analyzed the degree of lipid peroxidation in the mitochondria by measuring the amount of TBARS (thiobarbituric acid reactive substances). The amount of TBARS in heat-treated, no Ki-exposed mitochondria was greater than that of the control (no heat-treated, no Ki-exposed). However, the amount was reduced in the heat-treated, Ki-exposed mitochondria (two experiments; both P < 0.05) suggesting that Ki-energy protected mitochondria from oxidative stress. Calcium ions may play an important role in the protection by Ki-energy. Data also suggest that the observed Ki-effect involves, at least, near-infrared radiation (0.8-2.7 µm) from the human body.
Received October 19, 2005
Accepted May 11, 2006
Original Article
Ki-Energy (Life-Energy) Protects Isolated Rat Liver Mitochondria from Oxidative Injury
S. Tsuyoshi Ohnishi 1 *,
Tomoko Ohnishi 2,
and
Kozo Nishino 3
2 Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
3 School of Nishino Breathing Method, Shibuya-ku, Tokyo 150, Japan
S. Tsuyoshi Ohnishi, E-mail: stohnishi{at}aol.com
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