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eCAM Advance Access published online on April 24, 2006
eCAM, doi:10.1093/ecam/nel011
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© The Author (2006). Published by Oxford University Press. All rights reserved.
Received July 5, 2005
Accepted February 23, 2006

Original Article

Evaluation of Cytotoxic Effects of Dichloromethane Extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on Cultured HeLa Cells

Ganesh Chandra Jagetia 1 * and Shaival Kamalaksha Rao 1

1 Department of Radiobiology, Kasturba Medical College, Manipal 576104, India

* To whom correspondence should be addressed.
Ganesh Chandra Jagetia, E-mail: gc.jagetia{at}gmail.com


  Abstract

Extracts of Tinospora cordifolia (TCE) have been shown to possess anti-tumor properties, but the mechanism of the anti-tumor function of TCE is poorly understood. This investigation elucidates the possible mechanism underlying the cytotoxic effects of dichlormethane extracts of TCE, after selecting optimal duration and concentration for treatment. HeLa cells were exposed to various concentrations of TCE, which has resulted in a concentration-dependent decline in the clonogenicity, glutathione-S-transferase (GST) activity and a concentration-dependent increase in lipid peroxidation (TBARS) with a peak at 4 h and lactate dehydrogenase (LDH) release with a peak at 2 h. Our results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

Keywords: cytotoxicity; glutathione-S-transferase; Guduchi; HeLa; lactate dehydrogenase; lipid peroxidation.
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