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eCAM 2007 4(Supplement 1):21-23; doi:10.1093/ecam/nem109
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RAPD Analysis for Determination of Components in Herbal Medicine

V. M. Shinde1, K. Dhalwal1, K. R. Mahadik1, K. S. Joshi2 and B. K. Patwardhan2

1Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038 and 2Interdisciplinary School of Health Sciences, University of Pune, Pune 411007, Maharashtra, India

In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription, Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem of Tinospora cordifolia, dried fruit of Emblica officinalis and dried fruit of Tribulus terestris, the Ayurvedic prescription. Primer OPC-6 simultaneously generated three distinct amplicons, each specific to one component. The marker with 600 bp is specific to Tinospora cordifolia; the marker 500 bp is specific to Emblica officinalis and the remaining marker >1000 bp was present in Tribulus terestris. Presence of three herbal medicines was determined when RAPD reaction with OPC-6 was performed. The technique was proved to contribute to the identification of components in Ayurvedic herbal preparation and thus helping to serve as a complementary tool for quality control.

Keywords: DNA fingerprinting – herbal medicine – RAPD – standardization

For reprints and all correspondence: Vaibhav Shinde, Dept. of Pharmacognosy, Poona College of Pharmacy, Erandawane, Pune-411038. Maharashtra, India. Tel: +91-20-25437237, +91-20-25389728; Fax: +91-20-25439383; E-mail: vaibhavshinde2{at}rediffmail.com


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