Growth Inhibition of Cultured Human Liver Carcinoma Cells by Ki-energy (Life-energy): Scientific Evidence for Ki-effects on Cancer Cells
1Philadelphia Biomedical Research Institute Radnor, PA 19087, USA, 2Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine Phila, PA 19104, USA, 3School of Nishino Breathing Method Shibuya-ku, Tokyo 150-0002, and 4Graduate School of Nutritional Sciences, University of Shizuoka Yada, Shizuoka 422-8526, Japan
‘Ki-energy’ (life-energy) is believed to increase the immune activity of its practitioners. It has also been shown to cause neuropsychological effects. We undertook this study to obtain objective and scientific evidence as to whether or not a ‘Ki-effect’ could inhibit the growth of cultured cancer cells. Cultured human liver carcinoma cells, HepG2, were used. A Japanese Ki-expert held his fingers toward the cells in culture dishes for 5 or 10 min. After culturing for 24 h, we measured cell numbers, protein concentration per cell, certain mRNA expressions and the synthesis of regucalcin. The results were compared with those for control cells (non-treated cells). We found that the number of cells in the Ki-exposed groups were less than those in the controls by 30.3 and 40.6% with 5 and 10 min Ki-exposure, respectively. The protein content per cell in the Ki-exposed groups (5 and 10 min) was higher than that in the control groups by 38.8 and 62.9%, respectively. These results were statistically significant. Using RT–PCR, we found that the mRNA expression for c-myc, a tumor stimulator gene, was decreased, while that for regucalcin, which suppresses DNA synthesis, was increased. Our molecular biological studies and mathematical model analysis demonstrated that Ki-energy inhibited cancer cell division. The data also indicate that the Ki-effects involve some form of infrared radiation from the human body. This study suggests the possibility that Ki-energy may be beneficial for cancer patients because it suppresses cancer cell growth, and at the same time, it stimulates immune functions of the patients.
Keywords: breathing exercise – c-myc – cancer cells – HepG2 – infrared radiation – regucalcin
*For reprints and all correspondence: S. Tsuyoshi Ohnishi, PhD, Philadelphia Biomedical Research Institute, 502 King of Prussia Road, Radnor, PA 19087, USA. Tel: +1-610-688-6276; Fax: +1-610-254-9332; E-mail: stohnishi{at}aol.com
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