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eCAM Advance Access published online on October 29, 2009
eCAM, doi:10.1093/ecam/nep155
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Therapeutic Potential of Polar and Non-polar Extracts of Cyanthillium cinereum In vitro

Gunjan Guha, V. Rajkumar, R. Ashok Kumar and Lazar Mathew

School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu, India

Cyanthillium cinereum (Less.) H. Rob. (Asteraceae) has been traditionally known for its medicinal properties, all aspects of which are yet to be exploited. This study was aimed at investigating the therapeutic potential of polar (methanolic and aqueous) and nonpolar (hexane and chloroform) crude extracts of the whole plant. Several parameters including free-radical (DPPH•, ABTS•+, H2O2 and •OH) scavenging, reducing power, protection of DNA against oxidative damage, cytotoxicity, inhibition of oxidative hemolysis in erythrocytes, total phenolic content and inhibition of lipid peroxidation were examined. All the free-radical generating assay models demonstrated positive scavenging efficiency with differential but considerable magnitudes for the four extracts. However, only the hexane extract showed significant H2O2 scavenging effect. Lipid peroxidation was estimated by thiobarbituric acid–malondialdehyde (MDA) reaction, and a high degree of inhibition was shown by all the extracts. Reducing power of the polar extracts was higher than the non-polar ones. All extracts showed a concentration-dependent increase in phenolic contents. Oxidative damage to erythrocytes was hindered by all extracts in diverse degrees. XTT assay showed that all extracts have mild cytotoxic property. The aqueous extract evidently demonstrated protective effect on pBR322 plasmid DNA against oxidative breakdown. These results suggested the potential of C. cinereum as medicine against free-radical-associated oxidative damage and related degenerative diseases involving metabolic stress, genotoxicity and cytotoxicity.

Keywords: antioxidant – cytotoxicity – DNA damage inhibitor – free radical scavenger – lipid peroxidation

For reprints and all correspondence: R. Ashok Kumar, School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore 632 014, Tamil Nadu, India. Tel: +91-416-2202409; Fax:+91-416-2243092; E-mail: ashoku_2000{at}yahoo.com

Received January 27, 2009; accepted September 8, 2009


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