Skip Navigation



eCAM Advance Access published online on October 27, 2009
eCAM, doi:10.1093/ecam/nep151
This Article
Right arrow Full Text Freely available
Right arrow Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Nagai, T.
Right arrow Articles by Yamada, H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagai, T.
Right arrow Articles by Yamada, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Proteomic Analysis of Anti-inflammatory Effects of a Kampo (Japanese Herbal) Medicine ‘Shoseiryuto (Xiao-Qing-Long-Tang)’ on Airway Inflammation in a Mouse Model

Takayuki Nagai1,2, Marino Nakao1,*, Yuliko Shimizu1,*, Yoshio Kodera3, Masamichi Oh-Ishi3, Tadakazu Maeda3 and Haruki Yamada1,2

1Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, 2Oriental Medicine Research Center, Kitasato University, Tokyo and 3Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science, Sagamihara, Kanagawa, Japan

Effects of a Kampo (Japanese herbal) medicine ‘shoseiryuto (SST, xiao-qing-long-tang in Chinese)’, which has been used for the treatment of allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized allergic airway inflammation model (i.e. bronchial asthma) in a mouse. When SST was orally administered at 0.5 g kg–1 day–1 from day 1 to 6 after OVA inhalation, SST reduced the inflammation in lung tissue, the number of eosinophils and the OVA-specific immunoglobulin E (IgE) antibody titer in bronchoalveolar lavage (BAL) fluids at 7 days after the OVA inhalation. SST also reduced the airway hyperreactivity at 6 days after the OVA inhalation. Proteomic analysis with the agarose two-dimensional electrophoresis showed that the expression of spectrin {alpha}2 was reduced in the lung tissue of OVA-sensitized mice and SST recovered the expression. Western blot and immunohistochemical analyses of lung tissue also confirmed this result. When prednisolone was orally administered at 3 mg kg–1 day–1 from day 1 to 6 after OVA inhalation, the inflammation in lung tissue, the number of eosinophils in BAL fluids and airway hyperreactivity were reduced in the OVA-sensitized mice. However, prednisolone did not reduce the OVA-specific IgE antibody titer in BAL fluids and did not recover the expression of spectrin {alpha}2 in lung tissue. These results suggest that at least a part of action mechanism of SST against OVA-sensitized allergic airway inflammation in a mouse model is different from that of prednisolone.

Keywords: asthma – airway inflammation – eosinophil – proteomic analysis – shoseiryuto – spectrin {alpha}2 – xiao-qing-long-tang

For reprints and all correspondence: Haruki Yamada, Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan. Tel: +81-3-5791-6364; Fax: +81-3-3445-1351; E-mail: yamada{at}lisci.kitasato-u.ac.jp

*These authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.