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eCAM Advance Access published online on August 20, 2009
eCAM, doi:10.1093/ecam/nep121
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Full-length Sequence of Mouse Acupuncture-induced 1-L (Aig1l) Gene Including its Transcriptional Start Site

Mika Ohta1,2, Aki Sugano1, Shuji Goto3, Surini Yusoff4, Yushi Hirota5, Kotaro Funakoshi1, Kenji Miura1, Eiichi Maeda1, Nobuo Takaoka6, Nobuko Sato2, Hiroshi Ishizuka7, Naoki Arizono8, Hisahide Nishio4 and Yutaka Takaoka1,2

1Laboratory for Applied Genome Science and Bioinformatics, Clinical Genome Informatics Centre, Kobe University Graduate School of Medicine, Kobe 650-0017, 2Department of Biochemistry, Iwate Medical University School of Dentistry, Morioka 020-8505, 3Department of Acupuncture Informatics, Goto College of Medical Arts and Sciences, Tokyo 143-0016, 4Department of Genetic Epidemiology, 5Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, 6Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, 7Department of Anatomy, Tokushima University School of Dentistry, Tokushima 770-8504 and 8Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

We have been investigating the molecular efficacy of electroacupuncture (EA), which is one type of acupuncture therapy. In our previous molecular biological study of acupuncture, we found an EA-induced gene, named acupuncture-induced 1-L (Aig1l), in mouse skeletal muscle. The aims of this study consisted of identification of the full-length cDNA sequence of Aig1l including the transcriptional start site, determination of the tissue distribution of Aig1l and analysis of the effect of EA on Aig1l gene expression. We determined the complete cDNA sequence including the transcriptional start site via cDNA cloning with the cap site hunting method. We then analyzed the tissue distribution of Aig1l by means of northern blot analysis and real-time quantitative polymerase chain reaction. We used the semiquantitative reverse transcriptase-polymerase chain reaction to examine the effect of EA on Aig1l gene expression. Our results showed that the complete cDNA sequence of Aig1l was 6073 bp long, and the putative protein consisted of 962 amino acids. All seven tissues that we analyzed expressed the Aig1l gene. In skeletal muscle, EA induced expression of the Aig1l gene, with high expression observed after 3 h of EA. Our findings thus suggest that the Aig1l gene may play a key role in the molecular mechanisms of EA efficacy.

Keywords: cDNA cloning – electroacupuncture – molecular genetics

For reprints and all correspondence: Yutaka Takaoka, Division of Applied Genome Science and Bioinformatics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan. Tel: +81-78-382-5111 (ext. 2765); Fax: +81-78-382-5839; E-mail: ytakaoka{at}med.kobe-u.ac.jp

Received November 21, 2008; accepted July 21, 2009


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