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eCAM Advance Access published online on September 7, 2009
eCAM, doi:10.1093/ecam/nep119
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation

Beatriz Cesar1, Ana Paula R. Abud1, Carolina C. de Oliveira1, Francolino Cardoso2, Raffaello Popa Di Bernardi1, Fernando S.F. Guimarães1, Juarez Gabardo1 and Dorly de Freitas Buchi1

1Departamento de Biologia Celular, Setor de Ciências Biológicas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil and 2Instituto de Tecnologia do Paraná, Tecpar, Curitiba, Paraná, Brazil

A homeopathic complex medication (HCM), with immunomodulatory properties, is recommended for patients with depressed immune systems. Previous studies demonstrated that the medication induces an increase in leukocyte number. The bone marrow microenvironment is composed of growth factors, stromal cells, an extracellular matrix and progenitor cells that differentiate into mature blood cells. Mice were our biological model used in this research. We now report in vivo immunophenotyping of total bone marrow cells and ex vivo effects of the medication on mononuclear cell differentiation at different times. Cells were examined by light microscopy and cytokine levels were measured in vitro. After in vivo treatment with HCM, a pool of cells from the new marrow microenvironment was analyzed by flow cytometry to detect any trend in cell alteration. The results showed decreases, mainly, in CD11b and TER-119 markers compared with controls. Mononuclear cells were used to analyze the effects of ex vivo HCM treatment and the number of cells showing ring nuclei, niche cells and activated macrophages increased in culture, even in the absence of macrophage colony-stimulating factor. Cytokines favoring stromal cell survival and differentiation in culture were induced in vitro. Thus, we observe that HCM is immunomodulatory, either alone or in association with other products.

Keywords: bone marrow cells – macrophage activation – mononuclear cells – stromal cells

For reprints and all correspondence: Prof. Dorly de F. Buchi, UFPR, Centro Politécnico, SCB, Depto de Biologia Celular, sala 215, Laboratório de Estudo de Células Inflamatórias e Neoplásicas, Jardim das Américas, Curitiba, CEP 81531-980, PR, Brazil. Tel: +55-41-3361-1770; Fax: +55-41-3266-2042; E-mail: buchi{at}ufpr.br; labbiocel{at}ufpr.br

Received August 18, 2008; accepted July 21, 2009


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