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eCAM Advance Access published online on September 7, 2009
eCAM, doi:10.1093/ecam/nep109
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© The Author(s) 2009. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Phytochemical Composition and Antioxidant Capacity of Three Malian Medicinal Plant Parts

François Muanda1, Donatien Koné1, Amadou Dicko1, Rachid Soulimani2 and Chafique Younos2

1Chemistry Laboratory and Methodologies for the Environment, Paul-Verlaine University and 2Ethnobotanic, Pharmacology, Anxiety, Stress Oxidative and Bioactivity Laboratory, University P. Verlaine-Metz, Metz, France

This study evaluates the levels of total polyphenolic compounds in three Malian medicinal plants and determines their antioxidant potential. Quantitative and qualitative analysis of polyphenolics contained in plants extracts were carried out by RP-C18 RP–HPLC using UV detector. The antioxidant activity was determined by three tests. They are phosphomolybdenum, DPPH (2,2-diphenyl-1 picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic)] tests. The total phenolic and the total flavonoid contents varied from 200 to 7600 mg 100 g–1 dry weight (dw), expressed as gallic acid equivalents and from 680 to 12 300 mg 100 g–1 dw expressed as catechin equivalents, respectively. The total anthocyanin concentrations expressed as cyanin-3-glycoside equivalent varied from 1670 to 28 388 mg 100 g–1 dw. The antioxidant capacity was measured by determining concentration of a polyphenolic (in mg ml–1) required to quench the free radicals by 50% (IC50) and expressed as vitamin C equivalent antioxidant capacity. The IC50 values were ranked between 2.68 and 8.80 µg ml–1 of a solution of 50% (v/v) methanol in water. The uses of plants are rationalized on the basis of their antioxidant capacity.

Keywords: anthocyanins – flavonoids – Malian herbs – polyphenolic compounds – RP–HPLC

For reprints and all correspondence: A. Dicko, Chemistry Laboratory and Methodologies for the Environment, Paul-Verlaine University, 1, Bd. Arago Technopole 2000, 57078 Metz, France. Tel: +33-3-8731-5431; Fax: +33-3-8737-8506; E-mail: dicko{at}univ-metz.fr

Received October 17, 2008; accepted July 22, 2009


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