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eCAM Advance Access published online on June 24, 2009
eCAM, doi:10.1093/ecam/nep061
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Podophyllum hexandrum-Mediated Survival Protection and Restoration of Other Cellular Injuries in Lethally Irradiated Mice

Sanghmitra Sankhwar1, Manju Lata Gupta1, Vanita Gupta1, Savita Verma1, Krishna Avtar Suri3, Memita Devi2, Punita Sharma3, Ehsan Ahmed Khan4 and M. Sarwar Alam5

1Division of Radiation Biosciences,2NMR Research Center, Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Marg, New Delhi 110054,3Natural Products Chemistry Division, IIIM (CSIR), Canal Road, Jammu 180001,4Department of Medical Elementology and Toxicology and 5Department of Chemistry, Jamia Hamdard, Hamdard University, New Delhi 110062, India

This study aims at the development of a safe and effective formulation to counter the effects of lethal irradiation. The sub-fraction (G-001M), prepared from Podophyllum hexandrum has rendered high degree of survival (>90%) at a dose of 6 mg kg–1 body weight (intramuscular) in lethally irradiated mice. Therapeutic dose of G-001M, at about 20 times lower concentration than its LD100, has revealed a DRF of 1.62. Comet assay studies in peripheral blood leukocytes have reflected that, treatment of G-001M before irradiation has significantly reduced DNA tail length (P < 0.001) and DNA damage score (P < 0.001), as compared to radiation-only group. Spleen cell counts in irradiated animals had declined drastically at the very first day of exposure, and the fall continued till the 5th day (P < 0.001). In the treated irradiated groups, there was a steep reduction in the counts initially, but this phase did not prolong. More than 60% decline in thymocytes of irradiated group animals was registered at 5 h of irradiation when compared with controls, and the fall progressed further downwards with the similar pace till 5th day of exposure (P < 0.001). At later intervals, thymus was found fully regressed. In G-001M pre-treated irradiated groups also, thymocytes decreased till the 5th day but thereafter rejuvenated and within 30 days of treatment the values were close to normal. Current studies have explicitly indicated that, G-001M in very small doses has not only rendered high survivability in lethally irradiated mice, but also protected their cellular DNA, besides supporting fast replenishment of the immune system.

Keywords: Podophyllum hexandrum – DNA damage – radioprotector – thymocytes – splenocytes

For reprints and all correspondence: Dr Manju Lata Gupta, Sc ‘F’, Department of Radiation Biosciences, Institute of Nuclear Medicine & Allied Sciences, Brig. S. K. Majumdar Marg, Timarpur, New Delhi 110054, India. Tel: +91-11-23905153; Fax: +91-11-23919509; E-mail: drmanjugupta2003{at}yahoo.com

Received September 24, 2008; accepted May 7, 2009


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