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eCAM Advance Access published online on April 17, 2009
eCAM, doi:10.1093/ecam/nep031
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention

Sherry Soliman1, William J. Aronson2 and R. James Barnard1

1Department of Physiological Science and 2Department of Urology, Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA

Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for these anticancer effects. Again, the intervention slowed growth and increased apoptosis in LNCaP cells; responses that were eliminated when IGF-I was added back to the post-intervention samples. The p53 protein content was increased and NFkB activation reduced in the post serum-stimulated LNCaP cells. Similar results were observed when the IGF-I receptor was blocked in the pre-intervention serum. In androgen-independent PC-3 cells, growth was reduced while none of the other factors were changed by the intervention. We conclude that diet and exercise intervention might help prevent clinical PCa as well as aid in the treatment of PCa during the early stages of development.

Keywords: apoptosis – IGF-I – LNCaP – NFkB – p53 – PC-3

For reprints and all correspondence: R. James Barnard, Physiological Science, UCLA, 621 Charles E. Young Dr. So., Los Angeles, CA 90095, USA. Tel: +1-310-825-3794; Fax: +1-310-206-9184; E-mail: jbarnard{at}physci.ucla.edu

Received October 9, 2008; accepted March 27, 2009


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