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eCAM Advance Access published online on March 22, 2009
eCAM, doi:10.1093/ecam/nep021
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gingyo-San Enhances Immunity and Potentiates Infectious Bursal Disease Vaccination

Che-Ming Hung1,2,*, Chia-Chou Yeh3, Kowit-Yu Chong4,*, Hsiao-Ling Chen5,*, Jiun-Yu Chen2, Shung-Te Kao6, Chih-Ching Yen2,6, Ming-Hsien Yeh3, Maw-Sun Lin2 and Chuan-Mu Chen2

1Animal Industry Division, Livestock Research Institute, Council of Agriculture, Tainan 712, 2Department of Life Sciences, National Chung Hsing University, Taichung 402, 3Department of Chinese Medicine, Buddhist Dalin Tzu Chi General Hospital, Chia-Yi 622, 4Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Tao-Yuan 333, 5Department of Molecular Biotechnology, Da-Yeh University, Changhwa 515 and 6China Medical University Hospital, Taichung 404, Taiwan, ROC

The purpose of the present study was to investigate the effects of Gingyo-san (GGS), a traditional Chinese medical formula, on peripheral lymphocyte proliferation and serum antibody titers in chickens vaccinated against the infectious bursal disease (IBD) virus. Treatment groups were fed one of three doses of GGS in their diet (0.5%, 1.0% and 2.0%, w/w), and the IBD vaccine was administered at 1 and 3 weeks of age. At Weeks 8, 12 and 16, changes in serum IBD antibody titers were measured via the micro-method and T cell proliferation. In gene expression experiments, GGS-treated peripheral T lymphocytes were stimulated with concanavalin A (ConA) for 24 h. The mRNA expression of interleukin-2 (IL-2), interferon-{gamma} (IFN-{gamma}), interleukin-4 (IL-4) and interleukin-12 (IL-12) was determined using a semi-quantitative RT–PCR assay. The results showed that a low dose of GGS could significantly raise the antibody titers. Medium and high doses of GGS enhanced IL-2 and IFN-{gamma} production. GGS altered the expression of IL-4 and IL-12 in T lymphocytes. CD4+ T lymphocyte development was also skewed towards the Th1 phenotype. GGS enhanced cell-mediated immunity and augmented the effects of IBD vaccination in strengthening subsequent anti-viral responses.

Keywords: chlorogenic acid – Gingyo-San – infectious bursal disease – lymphocyte proliferation – serum antibody titer

For reprints and all correspondence: Chuan-Mu Chen, PhD, Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Road, Taichung 402, Taiwan, ROC. Tel: +886-4-22856309; Fax: +886-4-22851797; E-mail: chchen1{at}dragon.nchu.edu.tw

*These authors contributed equally to this work

Received July 16, 2008; accepted January 28, 2009


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