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eCAM Advance Access published online on October 15, 2008
eCAM, doi:10.1093/ecam/nen062
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Inhibition of cell growth and cellular protein, DNA and RNA synthesis in human hepatoma (HepG2) cells by ethanol extract of abnormal Savda Munziq of traditional Uighur medicine

Halmurat Upur1, Abdiryim Yusup1, Isabelle Baudrimont2, Anwar Umar1, Benedicte Berke1,3, Dilxat Yimit1, Jaya Conser Lapham1, Edmon E. Creppy2 and Nicholas Moore1,4

1Faculty of Traditional Uighur Medicine, Xinjiang Medical University, 830011 Urumqi, China, 2Department of Toxicology, 3Department of Pharmacognosy and 4Department of Pharmacology, University Victor Segalen Bordeaux 2, 33076 Bordeaux, France

Abnormal Savda Munziq (ASMq) is a traditional Uighur medicinal herbal preparation, commonly used for the treatment and prevention of cancer. We tested the effects of ethanol extract of ASMq on cultured human hepatoma cells (HepG2) to explore the mechanism of its putative anticancer properties, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide, neutral red and lactate dehydrogenase (LDH) leakage assays, testing the incorporation of 3[H]-leucine and 3[H]-nucleosides into protein, DNA and RNA, and quantifying the formation of malondialdehyde–thiobarbituric acid (MDA) adducts. ASMq ethanol extract significantly inhibited the growth of HepG2 and cell viability, increased the leakage of LDH after 48 h or 72 h treatment, in a concentration- and time-dependent manner (P < 0.05). Cellular protein, DNA and RNA synthesis were inhibited in a concentration- and time-dependent manner (P < 0.05). No significant MDA release in culture medium and no lipid peroxidation in cells were observed. The results suggest that the cytotoxic effects of ASMq ethanol extract might be related to inhibition of cancer cell growth, alteration of cell membrane integrity and inhibition of cellular protein, DNA and RNA synthesis.

Keywords: Abnormal Savda Munziq – cancer – cytotoxicity – herbal preparation – human hepatoma cells – traditional Uighur medicine

For reprints and all correspondence: Dr Abdiryim Yusup, Faculty of Traditional Uighur Medicine, Xinjiang Medical University, 393 Xinyi Road, 830011 Urumqi, Xinjiang, China. Tel/Fax: +86-991-43-66-014; E-mail: abdiryim{at}hotmail.com

Received June 20, 2008; accepted September 11, 2008


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