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eCAM Advance Access published online on June 12, 2008
eCAM, doi:10.1093/ecam/nen038
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Anti-oxidant and Anti-hypercholesterolemic Activities of Wasabia japonica

Young Sun Lee1,3, Jae Ha Yang2, Man Jong Bae2, Wang Keun Yoo2, Shen Ye3, Charlie C. L. Xue3 and Chun Guang Li3

1Department of Traditional Health Resource Development, College of Sung Duk, Yeongcheonsi, Gyeongsangbukdo 770-811, 2Daegu Haany University, Daegu 706-060, Korea and 3The RMIT Chinese Medicine Research Group, RMIT University, Bundoora West, Victoria 3083, Australia

The effects of Wasabia japonica (WJ) were investigated in vitro and in vivo for their anti-oxidant and anti-hypercholesterolemic activities. It was found that the aqueous extracts of WJ leaves (WJL) had strong scavenging activities towards 1,1-Diphenyl-2-picryhydrazyl (DPPH) and nitric oxide (NO) free radicals in cell free systems. WJL also inhibited NO production and the expressions of inducible NO synthase (iNOS) mRNA and enzyme protein, determined by Griess reactions, RT-PCR or Western blotting respectively in Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages cells. The anti-hypercholesterolemic effects of WJ diet were investigated in hypercholesterolemia rats. Sprague-Dawley rats were divided into four groups and were fed with either normal diet (Group 1), or diet containing 1%(w/w) cholesterol (Groups 2, 3 and 4). After 4 weeks, Group 2 was changed to normal diet, Groups 3 and 4 were changed to the diet containing 5% WJ leaf and or 5% WJ root, respectively. 3 weeks after WJ diets, Serum HDL-cholesterol levels were significantly increased in WJ diet groups compared with the normal diet hypercholesterolemia rats. In contrast, the serum LDL-cholesterol levels and liver xanthine oxidase (XO) activity in WJ diet groups were significantly decreased. The results indicate that the WJ extracts have significant anti-oxidant activities, and the WJ diet exhibited anti-hypercholesterolemic action in high cholesterol diet rats, which was companied with modulations of cholesterol metabolism and decrease in liver XO activity.

Keywords: anti-oxidant – cholesterol – hypercholesterolemic – Wasabia japonica

For reprints and all correspondence: Young Sun Lee, PhD, Department of Traditional Health Resource Development, College of Sung Duk, Yeongcheonsi Gyeongsangbukdo, 770-811, Republic of Korea, Tel: +82-54-330-8714; Fax: +82-54-330-8810; E-mail: yslee6784{at}yumail.ac.kr

Received June 28, 2007; accepted April 28, 2008


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