Skip Navigation



eCAM Advance Access published online on February 24, 2008
eCAM, doi:10.1093/ecam/nen010
This Article
Right arrow Full Text Freely available
Right arrow Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Shijie, Z.
Right arrow Articles by Kanda, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Shijie, Z.
Right arrow Articles by Kanda, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Mao-to Prolongs the Survival of and Reduces TNF-{alpha} Expression in Mice with Viral Myocarditis

Zhu Shijie1, Junji Moriya1, Jun’ichi Yamakawa1, Rui Chen1, Takashi Takahashi1, Hiroyuki Sumino2, Takeshi Nakahashi3, Kunimitsu Iwai3, Shigeto Morimoto3, Nobuo Yamaguchi4 and Tsugiyasu Kanda1

1Department of General Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, 2Department of Medicine and Biological Science, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, 3Department of Geriatric Medicine and 4Fundamental Research for Complementary and Alternative Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan

Goal of this study was to evaluate effects of Mao-to on development of myocarditis induced by encephalomyocarditis (EMC) virus in mice. Mice were randomly divided into five groups. Group N included uninfected controls (n = 18), while group A, B and C underwent intraperitoneal injection of EMC virus. Group A was administered oral saline from day 0 to day 4. Group B was administered oral Mao-to (500 mg–1 kg–1 day–1) from day 0 to day 4. Group C was administered Mao-to from day 2 to day 6. Group D was administered Mao-to from day 5 to day 10. Treated mice were followed for survival rates during 2 weeks after infection. Body weight (BW) and organ weights including heart (HW), lungs, thymus and spleen were examined on days 4, 6 and 14. Survival rate of group C (36.4%) was significantly improved compared with group A, B or D (0% of each, P < 0.05). HW and HW/BW ratio in group C was significantly (P < 0.05) lower than those in group A, B or D. Viral titers of hearts were significantly different among groups A, B and C. Cardiac expression in tumor necrosis factor-{alpha} (TNF-{alpha}) was significantly reduced in group C in comparison with group A, B or D on day 6 by immunohistochemical study. Administration of Mao-to starting on day 2 improves mortality resulting from viral myocarditis in mice with reduced expression of cardiac TNF-{alpha}. These findings suggest that timing of Mao-to is crucial for preventing cardiac damage in mice with viral myocarditis.

Keywords: Mao-to – viral myocarditis – tumor necrosis factor-{alpha}

For reprints and all correspondence: Tsugiyasu Kanda, MD, Department of General Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa, 920-0293, Japan. Tel: +81-76-286-2211 (ext. 3841); Fax: +81-76-286-2702; E-mail: kandat{at}kanazawa-med.ac.jp

Received February 15, 2007; accepted January 14, 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.