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eCAM Advance Access published online on November 14, 2007
eCAM, doi:10.1093/ecam/nem165
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

N-butanol Extract from Melilotus Suaveolens Ledeb Affects Pro- and Anti-Inflammatory Cytokines and Mediators

Lei Zhao1, Jun-Yan Tao2, Shu-Ling Zhang1, Feng Jin3, Ran Pang1 and Ji-Hua Dong4

1Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, 2College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430072, 3Department of Neurosurgery and 4Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P.R. China

Melilotus suaveolens Ledeb is a traditional medicinal plant for treating inflammation-related disease. This explores the inner anti-inflammatory mechanism of n-butanol extract from M. suaveolens Ledeb. Inflammatory cellular model was established by lipopolysaccharide intervention on RAW264.7 cell line. Levels of secreted cytokines TNF-{alpha}, IL-1ß, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-{alpha}, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-{kappa}B and ingredients in the extract were assayed by ELISA, real time quantitative PCR, western blot, immunocytochemical test and HPLC fingerprint test, respectively. As a result, the extract could not only markedly reduce the production of pro-inflammatory mediators to different extents by blocking NF-{kappa}B activation but also promote the release of anti-inflammatory mediator HO-1 significantly. Each 1 g extract contained 0.023531 mg coumarin and another two high polar ingredients, probably saponins. It can be concluded that the extract has similar effects on antagonizing pro-inflammatory mediators and cytokines like Dexamethasone, and has effects on promoting the production of anti-inflammatory mediators.

Keywords: Melilotus suaveolens Ledeb – n-butanol extract – RAW 264.7 cell line – NF-{kappa}B – HO-1

For reprints and all Correspondence: Prof. Shu-Ling Zhang, Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P.R. China. Tel: +86-27-62629209; Fax: +86-27-85756636; E-mail: chineseomd{at}yahoo.com The first two authors have contributed equally to this work.

Received June 18, 2007; accepted August 20, 2007


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