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eCAM Advance Access originally published online on November 1, 2007
eCAM 2009 6(2):233-238; doi:10.1093/ecam/nem099
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Regulation of Caspase-3 and Bcl-2 Expression in Dalton's Lymphoma Ascites Cells by Abrin

V. Ramnath1, P. S. Rekha1, G. Kuttan2 and R. Kuttan3

1Department of Physiology, College of Veterinary and Animal Sciences, Kerala Agricultural University, Mannuthy, Thrissur, 680 651, Kerala, 2Department of Immunology and 3Amala Cancer Research Centre, Amala Nagar, Thrissur, 680 553, Kerala, India

The role of abrin, a toxic lectin isolated from seeds of Abrus precatorius Linn in inducing apoptosis in murine Dalton's Lymphoma Ascites (DLA) cells was evaluated. Abrin when incubated at the concentration of 10 ng per million DLA cells could bring about cell death as typical morphological changes with apoptosis. However, necrotic cell death dominated when a higher dose of abrin was used. DNA samples, isolated from DLA cells treated with abrin showed fragmentation. Abrin brought about induction of apoptosis by stimulating the expression of pro-apoptotic Caspase-3, at the same time blocking the expression of Bcl-2, which is an anti apoptotic gene. However, the expression of tumor suppressor gene p53 has not been observed in control and abrin-treated DLA cells. Results suggested that abrin effectively induced apoptotic changes in the tumor cells that led to cellular death.

Keywords: abrin – apoptosis – Bcl-2 – Caspase-3 – p53

For reprints and all correspondence: Department of Physiology, College of Veterinary and Animal Sciences, Kerala Agricultural University, Mannuthy, Thrissur, 680 651, Kerala. Fax: 91-0487-2370388; Tel: 91-0487-2389057, E-mail: drvrnath{at}yahoo.com

Received October 17, 2006; accepted June 27, 2007


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