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eCAM Advance Access originally published online on August 23, 2007
eCAM 2008 5(1):107-113; doi:10.1093/ecam/nem002
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Exploring of Antimicrobial Activity of Triphala Mashi—an Ayurvedic Formulation

Yogesh S. Biradar1, Sheetal Jagatap2, K. R. Khandelwal1 and Smita S. Singhania3

1Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune 411 038, 2Department of Microbiology, University of Pune and 3Department of Management Sciences, University of Pune, Pune, Maharashtra, India

Triphala Mashi is an ayurvedic formulation that was prepared in our lab. Aqueous and alcoholic extracts of both Triphala and Triphala Mashi were used, to evaluate antimicrobial activity. Comparative phytochemical profile of Triphala and Triphala Mashi was done by preliminary phytochemical screening, total phenolic content and thin layer chromatography (TLC). Antimicrobial activity includes isolation of pathogens from clinical samples, its characterization, testing its multiple drug resistance against standard antibiotics and antimicrobial activity of aqueous and alcoholic extracts of both Triphala and Triphala Mashi against these organisms by using agar gel diffusion method. Triphala Mashi containing phenolic compounds, tannins exhibited comparable antimicrobial activity in relation to Triphala against all the microorganisms tested. It inhibits the dose-dependent growth of Gram-positive and Gram-negative bacteria. In conclusion, it appears that Triphala Mashi has non-specific antimicrobial activity.

Keywords: antimicrobial activity – clinical sample and Tannin – Triphala – Triphala Mashi

For reprints and all correspondence: Yogesh S. Biradar, Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune 411 038, India. Tel: +91-98792-294347; E-mail: biradar_yogesh{at}rediffmail.com

Received February 26, 2006; accepted September 26, 2006


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