Skip Navigation


eCAM Advance Access originally published online on December 5, 2006
eCAM 2007 4(3):335-341; doi:10.1093/ecam/nel097
This Article
Right arrow Full Text Freely available
Right arrow Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
4/3/335    most recent
nel097v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Nishida, S.
Right arrow Articles by Satoh, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishida, S.
Right arrow Articles by Satoh, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2006 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Vascular Pharmacology of Mokuboito (Mu-Fang-Yi-Tang) and Its Constituents on the Smooth Muscle and the Endothelium in Rat Aorta

Seiichiro Nishida and Hiroyasu Satoh

Department of Pharmacology, Division of Crude and Herbal Medicine, Nara Medical University Kashihara, Nara 634-8521, Japan

Pharmacological actions of Mokuboito and its constituents (Sinomenium acutum and sinomenine) on rat aorta were examined. Mokuboito and S. acutum at lower concentrations (0.03–1 mg ml–1) contracted the non-loaded aorta, but at higher concentrations (1–3 mg ml–1), reversed to dilate it. The vasoconstriction was blocked by phentolamine (10 µM). Sinomenine failed to exhibit the vasoconstriction. On the other hand, Mokuboito and S. acutum dilated the NE (5 µM)-induced vasoconstriction: at 3 mg ml–1, by 98.9 ± 2.5% (n = 6, P < 0.01) and 97.0 ± 4.8% (n = 6, P < 0.01). Vasorelaxation induced by Mokuboito and S. acutum was attenuated by indomethacin, L-NMMA and nicardipine. Propranolol decreased the vasorelaxation induced by Mokuboito, but not by S. acutum. Sinomenine also relaxed the constriction and at 100 µM, by 68.8 ± 5.1% (n = 7, P < 0.01). This vasorelaxation was attenuated by indomethacin, L-NMMA and nicardipine, and also by propranolol. Therefore, these results indicate that Mokuboito and its constituents exert both vasodilating actions mediated by endothelium-dependent mechanisms (PGI2 and NO from endothelium) and by endothelium-independent mechanisms (Ca2+ influx control on smooth muscle cells). Simultaneously, Mokuboito and S. acutum cause the vasoconstrictions mediated through {alpha}-adrenoceptor stimulation, but not sinomenine. Also, Mokuboito and sinomenine possess ß-adrenoreceptor stimulating action, but not S. acutum.

Keywords: {alpha}- and ß-adrenoceptors – Ca2+ channel – EDRF – endothelium – Mokuboito – PGI2 – rat aorta – sinomenine – Sinomenium acutum – vasodilation

For reprints and all correspondence: Dr Hiroyasu Satoh, Department of Pharmacology, Nara Medical University, Kashihara, Nara 634-8521, Japan. Tel: +81-744-29-8831; Fax: +81-744-29-0510; E-mail: hysat{at}naramed-u.ac.jp

Received February 22, 2006; accepted October 30, 2006


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.