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eCAM Advance Access originally published online on December 1, 2006
eCAM 2007 4(3):327-333; doi:10.1093/ecam/nel093
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© 2006 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Anti-Inflammatory Activity of the Methanol Extract of Moutan Cortex in LPS-Activated Raw264.7 Cells

Seung-Chul Chun, Seon Young Jee, Sang Gon Lee, Sook Jahr Park, Jong Rok Lee and Sang Chan Kim

College of Oriental Medicine and Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University Daegu 706-828, Republic of Korea

Moutan Cortex (MCE) has been used in traditional medicine to remove heat from the blood, promote blood circulation and alleviate blood stasis. This study was conducted to evaluate the effects of MCE on regulatory mechanisms of cytokines and nitric oxide (NO) involved in immunological activity of Raw264.7 cells. Cells were pretreated with methanolic extracts of MCE, and further cultured for an appropriate time after lipopolyssacharide (LPS) addition. During the entire experimental period, 0.1 and 0.3 mg ml–1 of MCE had no cytotoxicity. In these concentrations, MCE inhibited the production of NO and prostaglandin E2 (PGE2), the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor of {kappa}B{alpha} (p-I{kappa}B{alpha}), and the activation of nuclear factor {kappa}B (NF-{kappa}B). MCE also reduced the concentration of tumor necrosis factor-{alpha} (TNF-{alpha}), interleukin-1ß (IL-1ß) and interleukin-6 (IL-6) in the Raw264.7 cells that were activated by LPS. These results demonstrate that MCE has anti-inflammatory effects through the inhibition of iNOS and COX-2 expression by suppressing the phosphorylation of I-{kappa}B{alpha} and the activation of NF-{kappa}B.

Keywords: LPS – Moutan Cortex – NF-{kappa}B – nitric oxide

For reprints and all correspondence: Sang Chan Kim, College of Oriental Medicine, Daegu Haany University, 165 Sang-dong, Suseong-gu, Daegu 706-828, Republic of Korea. Tel: +82-53-770-2247; Fax: +82-53-768-6340; E-mail: sckim{at}dhu.ac.kr

Received June 18, 2006; accepted October 13, 2006


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