eCAM Advance Access originally published online on March 12, 2007
eCAM 2007 4(3):313-319; doi:10.1093/ecam/nem011
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Antioxidant Activity of Leaves and Fruits of Iranian Conifers
1Department of Pharmacognosy, School of Pharmacy, Biotechnology Research Center and 2Department of Medicinal Chemistry, School of Pharmacy, Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, 91775-1365, Iran
Cupressus semipervirens var. horizontalis, Cupressus semipervirens var. semipervirens, Cupressus semipervirens cv. Cereifeormis, Juniperus communis subsp. hemisphaerica, Juniperus excelsa subsp. excelsa, Juniperus excelsa subsp. polycarpos, Juniperus foetidissima, Juniperus oblonga, Juniperus sabina, Platycladus orientalis and Taxus baccata are Iranian conifers. The antioxidant activity of leaves and fruits of these 11 different taxons were evaluated. The leaves of both male and female, and fruits of these plants were collected from different areas of the country. Methanol extract of leaves and fruits of these taxons were prepared. Antioxidant activity of each extracts was measured using two different tests of the ferric thiocyanate method and thiobarbituric acid. Results indicated that the methanol extracts of leaves, of male and female, and fruits of all these species (27 samples) possessed antioxidant activity when tested with both methods. The antioxidant activity was then compared with those of
-tocopherol (a natural antioxidant) and butylated hydroxytoluene (a synthetic antioxidant). Methanol extract of fruits of C. semipervirens cv. Cereifeormis showed the highest antioxidant activity while the methanol extract of leaves of C. semipervirens var. semipervirens possessed the lowest antioxidant activity. However, our finding showed that most of the tested extracts were showing strong antioxidant activity even higher than
-tocopherol.
Keywords: antioxidative activity – conifers, Cupressus – ferric thiocyanate test – Juniperus – medicinal plant – Platycladus orientalis – radical scavenging – thiobarbituric acid test
For reprints and all correspondence: Prof. Mohammad Hassanzadeh-Khayyat, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 91775-1365, Iran. Tel: (0511) 8823255-65; Fax: (0511) 8823251; E-mail: hassanzadeh_mk{at}yahoo.com
Received May 28, 2006; accepted January 16, 2007
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