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eCAM Advance Access originally published online on September 11, 2006
eCAM 2007 4(1):83-94; doi:10.1093/ecam/nel059
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© 2006 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

Delayed Cell Cycle Progression and Apoptosis Induced by Hemicellulase-Treated Agaricus blazei

Masaki Kawamura1 and Hirotake Kasai2

1Department of Alternative Medicine and Bioregulation, Faculty of Medicine and 2Department of Microbiology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi Yamanashi, Japan

We examined the effects of hemicellulase-treated Agaricus blazei (AB fraction H, ABH) on growth of several tumor cell lines. ABH inhibited the proliferation of some cell lines without cytotoxic effects. It markedly prolonged the S phase of the cell cycle. ABH also induced mitochondria-mediated apoptosis in different cell lines. However, it had no impact on the growth of other cell lines. ABH induced strong activation of p38 mitogen-activated protein kinase (MAPK) in the cells in which it evoked apoptosis. On the other hand, ABH showed only a weak p38 activation effect in those cell lines in which it delayed cell cycle progression with little induction of apoptosis. However, p38 MAPK-specific inhibitor inhibited both ABH-induced effects, and ABH also caused apoptosis in the latter cells under conditions of high p38 MAPK activity induced by combined treatment with TNF-{alpha}. These results indicate that the responsiveness of p38 MAPK to ABH, which differs between cell lines, determines subsequent cellular responses on cell growth.

Keywords: antiproliferation – cancer – cell lines – fungus – p38 MAPK

For reprints and all correspondence: Dr M. Kawamura, Department of Alternative Medicine and Bioregulation, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan. Tel: +81-55-273-9539; Fax: +81-55-273-6728; E-mail: masakika{at}yamanashi.ac.jp

Received March 12, 2006; accepted August 10, 2006


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