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eCAM Advance Access originally published online on June 21, 2006
eCAM 2006 3(4):503-511; doi:10.1093/ecam/nel037
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© The Author (2006). Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans

Raman Chawla1, Rajesh Arora1, Shikha Singh1, R.K. Sagar1, Rakesh Kumar Sharma1,2, R. Kumar1, A. Sharma1, R. P. Tripathi1, S. C. Puri3, H. A. Khan4, A. S. Shawl5, P. Sultan5, Tej Krishan5 and G. N. Qazi3

1 Division of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences Brigadier SK Mazumdar Marg, Delhi 110054, 2 Defence Research Laboratory (DRDO) Tejpur, Assam, 3 Natural Products Chemistry Division, Regional Research Laboratory (CSIR) Jammu Tawi 180001, 4 Department of Medical Elementology and Toxicology Jamia Hamdard, Hamdard Nagar, Delhi 110062, and 5 Regional Research Laboratory (CSIR), Field Station Bonera, Srinagar, India

We have evaluated the effect of variation in aryl-tetralin lignans on the radioprotective properties of Podophyllum hexandrum. Two fractionated fractions of P. hexandrum [methanolic (S1) and chloroform fractions (S2)], with varying aryl-tetralin lignan content were utilized for the present study. The peroxyl ion scavenging potentials of S1 and S2 were found to be comparable [i.e. 45.88% (S1) and 41% (S2)] after a 48 h interval in a time-dependent study, whereas in a 2 h study, S2 exhibited significant (P < 0.05) antioxidant activity in different metal ion + flux states. In the aqueous phase, S2 exhibited non-site-specific reactive oxygen species scavenging activity, i.e. 73.12% inhibition at 500 µg ml–1. S1 exhibited 58.40 ± 0.8% inhibition (at 0.025 µg ml–1) of the formation of reactive nitrite radicals, comparable to S2 (52.45 ± 0.825%), and also showed 45.01% site-specific activity (1000 µg ml–1), along with significant (P < 0.05) electron donation potential (50–2000 µg ml–1) compared to S2. Such activities of S1 could be attributed to the significantly (P < 0.05) higher levels of podophyllotoxin ß-D-glucopyranoside (16.5 times) and demethyl podophyllotoxin glucoside (2.9 times) compared with S2. Together, these findings clearly prove that aryl-tetralin lignan content influences the radiation protective potential of the Podophyllum fractions to a great extent.

Keywords: antioxidant – aryl-tetralin lignans – biological radioprotection – hydroxyl ion podophyllotoxin glucopyranoside

Dr. Rakesh Kumar Sharma, Defence Research Laboratory, Tejpur, Assam; E-mail: rksharmadrl{at}yahoo.com; or Dr. Rajesh Arora, Institute of Nuclear Medicine and Allied Sciences, Brigadier. S.K. Mazumdar Road, Delhi-110054, India. Tel: 091-011-23905149; Fax: 091-011-2391950; E-mail: rajesharoradr{at}rediffmail.com


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This article has been cited by other articles:


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[Abstract] [PDF]


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