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eCAM Advance Access originally published online on June 8, 2006
eCAM 2006 3(4):475-482; doi:10.1093/ecam/nel032
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© The Author (2006). Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Ki-Energy (Life-Energy) Protects Isolated Rat Liver Mitochondria from Oxidative Injury

S. Tsuyoshi Ohnishi1, Tomoko Ohnishi2 and Kozo Nishino3

1 Philadelphia Biomedical Research Institute King of Prussia, PA 19406, 2 Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine Philadelphia, PA 19104, USA, and 3 School of Nishino Breathing Method, Shibuya-ku Tokyo 150, Japan

We investigated whether ‘Ki-energy’ (life-energy) has beneficial effects on mitochondria. The paradigm we developed was to keep isolated rat liver mitochondria in conditions in which they undergo heat deterioration (39°C for 10 min). After the heat treatment, the respiration of the mitochondria was measured using a Clarke-type oxygen electrode. Then, the respiratory control ratio (RC ratio; the ratio between State-3 and State-4 respiration, which is known to represent the integrity and intactness of isolated mitochondria) was calculated. Without the heat treatment, the RC ratio was >5 for NADH-linked respiration (with glutamate plus malate as substrates). The RC ratio decreased to 1.86–4.36 by the incubation at 39°C for 10 min. However, when Ki-energy was applied by a Japanese Ki-expert during the heat treatment, the ratio was improved to 2.24–5.23. We used five preparations from five different rats, and the significance of the differences of each experiment was either P < 0.05 or P < 0.01 (n = 3–5). We analyzed the degree of lipid peroxidation in the mitochondria by measuring the amount of TBARS (thiobarbituric acid reactive substances). The amount of TBARS in heat-treated, no Ki-exposed mitochondria was greater than that of the control (no heat-treated, no Ki-exposed). However, the amount was reduced in the heat-treated, Ki-exposed mitochondria (two experiments; both P < 0.05) suggesting that Ki-energy protected mitochondria from oxidative stress. Calcium ions may play an important role in the protection by Ki-energy. Data also suggest that the observed Ki-effect involves, at least, near-infrared radiation (0.8–2.7 µm) from the human body.

Keywords: infrared radiation – Ki-energy – lipid peroxidation – mitochondria – oxidative stress – reactive oxygen species – respiratory control ratio – TBARS

For reprints and all correspondence: S. Tsuyoshi Ohnishi, PhD, Philadelphia Biomedical Research Institute, suite 250, 100 Ross Road, King of Prussia. PA 19406, USA. Tel: +1-610-688-6276; Fax: +1-610-254-9332; E-mail: stohnishi{at}aol.com


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