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eCAM Advance Access originally published online on April 24, 2006
eCAM 2006 3(2):267-272; doi:10.1093/ecam/nel011
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© The Author (2006). Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Evaluation of Cytotoxic Effects of Dichloromethane Extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on Cultured HeLa Cells

Ganesh Chandra Jagetia and Shaival Kamalaksha Rao

Department of Radiobiology, Kasturba Medical College Manipal 576104, India

Extracts of Tinospora cordifolia (TCE) have been shown to possess anti-tumor properties, but the mechanism of the anti-tumor function of TCE is poorly understood. This investigation elucidates the possible mechanism underlying the cytotoxic effects of dichlormethane extracts of TCE, after selecting optimal duration and concentration for treatment. HeLa cells were exposed to various concentrations of TCE, which has resulted in a concentration-dependent decline in the clonogenicity, glutathione-S-transferase (GST) activity and a concentration-dependent increase in lipid peroxidation (TBARS) with a peak at 4 h and lactate dehydrogenase (LDH) release with a peak at 2 h. Our results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

Keywords: cytotoxicity – glutathione-S-transferase – Guduchi – HeLa – lactate dehydrogenase – lipid peroxidation

For reprints and all correspondence: Dr Ganesh Chandra Jagetia, Professor and Head, Department of Radiobiology, Kasturba Medical College, Manipal 576104, India. Tel: +91-820-2922122; Fax: +91-820-2571919; E-mail: gc.jagetia{at}gmail.com


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